On top of biosynthetic gene clusters: How epigenetic machinery influences secondary metabolism in fungi
نویسندگان
چکیده
منابع مشابه
Next-generation sequencing approach for connecting secondary metabolites to biosynthetic gene clusters in fungi
Genomics has revolutionized the research on fungal secondary metabolite (SM) biosynthesis. To elucidate the molecular and enzymatic mechanisms underlying the biosynthesis of a specific SM compound, the important first step is often to find the genes that responsible for its synthesis. The accessibility to fungal genome sequences allows the bypass of the cumbersome traditional library constructi...
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Although biosynthetic gene clusters (BGCs) have been discovered for hundreds of bacterial metabolites, our knowledge of their diversity remains limited. Here, we used a novel algorithm to systematically identify BGCs in the extensive extant microbial sequencing data. Network analysis of the predicted BGCs revealed large gene cluster families, the vast majority uncharacterized. We experimentally...
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Biosynthetic pathways of secondary metabolites from fungi are currently subject to an intense effort to elucidate the genetic basis for these compounds due to their large potential within pharmaceutics and synthetic biochemistry. The preferred method is methodical gene deletions to identify supporting enzymes for key synthases one cluster at a time. In this study, we design and apply a DNA expr...
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ژورنال
عنوان ژورنال: Biotechnology Advances
سال: 2019
ISSN: 0734-9750
DOI: 10.1016/j.biotechadv.2019.02.001